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KMID : 0380219930260020139
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Journal of Biochemistry and Molecular Biology
1993 Volume.26 No. 2 p.139 ~ p.144
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Immunological Relationships of Tobacco Isoperoxidases
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Mi Young Lee and Soung Soo Kim
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Abstract
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Two cationic isoperoxidases (termed C₃ and C₄) and two anionic isoperoxidases (termed A₃ and A₄) have been purified to homogeneity from a tobacco callus culture, Nicotiana tabacum L. var Virginia 115, utilizing ammonium sulfate precipitation, CM-cellulose chromatography, DEAE-cellulose chromatography followed by Sephadex G-100 gel filtration. Antisera against purified isoperoxidases were raised in rabbits, and the quantity of immune IgG which yielded half maximal enzyme inhibition was determined by immunotitration. In Ouchterlony double diffusion experiments, the cationic isoperoxidase C₃ and C₄ revealed an immunological identity, whereas these isoperoxidases had no immunological relationships with anionic isoperoxidase A₃ and A₄. Only anti A₄ antiserum cross-reacted with horseradish peroxidase preparation, although it did not cross-react with isoperoxidase A₃. These results suggest an immunological uniqueness of isoperoxidase A₃ among these tobacco isoperoxidases.
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